dsm 44186 Search Results


rhodococcus opacus pd630  (DSMZ)
94
DSMZ rhodococcus opacus pd630
Figure 2. CT values for ten candidate reference genes in R. opacus. RNA was extracted from biological triplicates of R. opacus grown in four distinct growth conditions (HN [circle], LN [triangle], TSB [diamond], and PHE [square]; see Materials and Methods for full description). RT-qPCR was performed in technical triplicate on each biological replicate. Each point represents the average threshold cycle (CT) value of all replicates for the listed gene and growth condition. Error bars represent one standard deviation.
Rhodococcus Opacus Pd630, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. CT values for ten candidate reference genes in R. opacus. RNA was extracted from biological triplicates of R. opacus grown in four distinct growth conditions (HN [circle], LN [triangle], TSB [diamond], and PHE [square]; see Materials and Methods for full description). RT-qPCR was performed in technical triplicate on each biological replicate. Each point represents the average threshold cycle (CT) value of all replicates for the listed gene and growth condition. Error bars represent one standard deviation.

Journal: Scientific reports

Article Title: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630.

doi: 10.1038/s41598-018-24486-w

Figure Lengend Snippet: Figure 2. CT values for ten candidate reference genes in R. opacus. RNA was extracted from biological triplicates of R. opacus grown in four distinct growth conditions (HN [circle], LN [triangle], TSB [diamond], and PHE [square]; see Materials and Methods for full description). RT-qPCR was performed in technical triplicate on each biological replicate. Each point represents the average threshold cycle (CT) value of all replicates for the listed gene and growth condition. Error bars represent one standard deviation.

Article Snippet: Rhodococcus opacus PD630 (DSMZ 44193) was grown in either a minimal salts medium as previously described (minimal media recipe B)9, with one of three distinct combinations of carbon and nitrogen sources (discussed in detail in the Gene expression studies section), or a rich tryptic soy broth (TSB) medium.

Techniques: Quantitative RT-PCR, Standard Deviation

Figure 5. Effect of reference gene choice on RT-qPCR normalization. (A) Box plots of averaged PD630_ RS15810 expression data (CT) for all four growth conditions (HN, LN, TSB, and PHE). Each gray box represents the first through third quartiles, the solid black line represents the median, and the whiskers represent the minimum and maximum values. (B–D) The normalized relative expression ratio of PD630_LPD05540 going from HN to either LN (B), TSB (C), or PHE (D). The expression data was normalized with a NF, including RG6, RG10, RG3/RG7, RG3/RG8, RG7/RG8, and RG3/RG7/RG8 using REST 2009. Error bars represent the 95% confidence interval (CI) as calculated by REST 2009. Stars indicate that a 95% CI range falls outside of the 95% CI range of the RG3/RG7/RG8 (and RG7/RG8) normalized ratio.

Journal: Scientific reports

Article Title: Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630.

doi: 10.1038/s41598-018-24486-w

Figure Lengend Snippet: Figure 5. Effect of reference gene choice on RT-qPCR normalization. (A) Box plots of averaged PD630_ RS15810 expression data (CT) for all four growth conditions (HN, LN, TSB, and PHE). Each gray box represents the first through third quartiles, the solid black line represents the median, and the whiskers represent the minimum and maximum values. (B–D) The normalized relative expression ratio of PD630_LPD05540 going from HN to either LN (B), TSB (C), or PHE (D). The expression data was normalized with a NF, including RG6, RG10, RG3/RG7, RG3/RG8, RG7/RG8, and RG3/RG7/RG8 using REST 2009. Error bars represent the 95% confidence interval (CI) as calculated by REST 2009. Stars indicate that a 95% CI range falls outside of the 95% CI range of the RG3/RG7/RG8 (and RG7/RG8) normalized ratio.

Article Snippet: Rhodococcus opacus PD630 (DSMZ 44193) was grown in either a minimal salts medium as previously described (minimal media recipe B)9, with one of three distinct combinations of carbon and nitrogen sources (discussed in detail in the Gene expression studies section), or a rich tryptic soy broth (TSB) medium.

Techniques: Quantitative RT-PCR, Expressing